Coding

Part:BBa_K1999001:Design

Designed by: Jimin Sun   Group: iGEM16_KoreaSonyeodul   (2016-10-10)


Mono(2-hydroxyethyl) terephthalate hydrolase (MHETase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1584
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1584
    Illegal NotI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
    Illegal BamHI site found at 424
    Illegal BamHI site found at 552
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1584
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1584
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 1138
    Illegal NgoMIV site found at 1543
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 279


Design Notes

N/A


Source

Ideonella sakaiensis (strain 201-F6)

References